Cell-Free DNA Integrity Analysis in Urine Samples
نویسندگان
چکیده
منابع مشابه
Urine Cell-Free DNA Integrity Analysis for Early Detection of Prostate Cancer Patients
INTRODUCTION The detection of tumor-specific markers in urine has paved the way for new early noninvasive diagnostic approaches for prostate cancer. We evaluated the DNA integrity in urine supernatant to verify its capacity to discriminate between prostate cancer and benign diseases of the urogenital tract. PATIENTS AND METHODS A total of 131 individuals were enrolled: 67 prostate cancer pati...
متن کاملUrine Cell-Free DNA Integrity as a Marker for Early Prostate Cancer Diagnosis: A Pilot Study
Circulating cell-free DNA has been recognized as an accurate marker for the diagnosis of prostate cancer, whereas the role of urine cell-free DNA (UCF DNA) has never been evaluated in this setting. It is known that normal apoptotic cells produce highly fragmented DNA while cancer cells release longer DNA. We thus verified the potential role of UCF DNA integrity for early prostate cancer diagnos...
متن کاملUrine cell-free DNA integrity as a marker for early bladder cancer diagnosis: preliminary data.
OBJECTIVES Urine cell-free (UCF) DNA has recently been proposed as a potential marker for early bladder cancer diagnosis. It is known that normal apoptotic cells produce highly fragmented DNA while cancer cells release longer DNA. Therefore, we verified the potential role of UCF DNA integrity in early bladder cancer diagnosis. MATERIALS AND METHODS UCF DNA was isolated from 51 bladder cancer ...
متن کاملOn targeting cell-free DNA in urine: a protocol for optimized DNA analysis.
This is not the first editorial in Clinical Chemistry dedicated to the testing of urine for cell-free DNA. In 2000 Lo (1 ) highlighted the findings of Botezatu et al. (2 ), who were the first to describe a transfer of DNA across the kidney barrier into urine. The origins of detected transrenal DNA sequences were carcinomas, blood transfusions, and, in pregnant women, the fetus. Following this f...
متن کاملPresence of donor- and recipient-derived DNA in cell-free urine samples of renal transplantation recipients: urinary DNA chimerism.
BACKGROUND Previous studies have indicated that microchimerism is present in body tissues, peripheral blood, and plasma of recipients after organ transplantation. We hypothesize that donor-derived DNA may also be present in cell-free urine of renal transplant recipients and that the concentrations of urine DNA may be correlated with graft rejection. METHODS Thirty-one female patients who had ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2017
ISSN: 1940-087X
DOI: 10.3791/55049